Wan X S, St Clair D K
Graduate Center for Toxicology, University of Kentucky, Lexington 40506-0054.
Arch Biochem Biophys. 1993 Jul;304(1):89-93. doi: 10.1006/abbi.1993.1325.
The effect of three thiol-modulating agents, buthionine sulfoximine (BSO), N-ethylmaleimide (NEM), and diazenedicarboxylic acid bis(N,N'-dimethylamide) (diamide), on cellular glutathione (GSH) level and manganese superoxide dismutase (MnSOD) activity was studied in a human lung fibroblast cell line, WI38. The results showed that treatment with BSO, NEM, or diamide can increase MnSOD activity in the human fibroblast cells. These findings are compatible with the view that alteration of intracellular GSH level may create a pro-oxidant environment in the cells and the MnSOD activity may increase as a cellular response to the oxidative stress.
在人肺成纤维细胞系WI38中研究了三种硫醇调节剂，即丁硫氨酸亚砜胺（BSO）、N-乙基马来酰亚胺（NEM）和二氮杂双羧酸双（N，N'-二甲基酰胺）（二酰胺）对细胞内谷胱甘肽（GSH）水平和锰超氧化物歧化酶（MnSOD）活性的影响。结果表明，用BSO、NEM或二酰胺处理可增加人成纤维细胞中的MnSOD活性。这些发现与以下观点一致，即细胞内GSH水平的改变可能在细胞中产生促氧化环境，并且MnSOD活性可能作为细胞对氧化应激的反应而增加。
