McCready P, Takagi M, Doi R H
Department of Biochemistry and Biophysics, University of California, Davis 95616.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):1110-5. doi: 10.1006/bbrc.1993.1740.
We have identified a stem-and-loop region between the promoter and open reading frame of the Bacillus subtilis senS gene containing a 9 base sequence (TTTAGATAA) identical to a sequence found in a similar regulatory element of the inducible pC194 cat gene. A high copy number of this pC194 regulatory region repressed the expression of senS, indicating that the stem-and-loop region of the cat gene titrated a positive factor necessary for senS expression. These results suggest that factors with similar binding requirements control senS and cat expression. In addition, senS was found to be autoregulated.
我们已在枯草芽孢杆菌senS基因的启动子和开放阅读框之间鉴定出一个茎环区域，该区域包含一段9个碱基的序列（TTTAGATAA），与诱导型pC194 cat基因的类似调控元件中发现的序列相同。该pC194调控区域的高拷贝数抑制了senS的表达，表明cat基因的茎环区域滴定了senS表达所需的一个正向因子。这些结果表明，具有相似结合需求的因子控制着senS和cat的表达。此外，还发现senS受到自身调控。
