Matsumoto K, Takao Y, Akazawa S, Yano M, Uotani S, Kawasaki E, Takino H, Yamasaki H, Okuno S, Yamaguchi Y
First Department of Internal Medicine, Nagasaki University School of Medicine, Japan.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):1275-82. doi: 10.1006/bbrc.1993.1763.
The developmental expression and localization of facilitative glucose transporter protein (GLUT-1, GLUT-2 and GLUT-5) in rat embryonal and fetal intestine were examined using immunohistochemical methods. The intestines were collected from embryo and fetus (term days, 10 to 20 of gestation), newborn and adult rats (16 weeks after birth). GLUT-1 was expressed abundantly in the gut tube on day 10 of gestation. From day 10 to birth, GLUT-1 was expressed continuously but its expression decreased gradually. In the adult, no GLUT-1 was detected. GLUT-2 was not expressed from day 10 to 14, but began to be expressed in the lumen of the gut tube on day 16, before the intestinal villi were evident. On day 18, when the intestinal villi were developing, GLUT-2 was localized to the basolateral membranes of the enterocytes. A similar pattern of GLUT-2 localization was also observed in newborn and adult rat intestines. The expression kinetics of GLUT-5 were almost the same as those of GLUT-2. GLUT-5 began to be expressed in the lumen of the gut tube on day 16. In contrast to GLUT-2, GLUT-5 was localized to the brush border membranes of intestinal villi after day 18. These results indicate that the expression and localization of each member of the glucose transporter family in the embryonal and fetal intestine vary with the gestational phase. GLUT-1 may be required for glucose metabolism early in gestation, and GLUT-2 and GLUT-5 may be required in both late gestational and perinatal stages.
采用免疫组化方法检测了大鼠胚胎和胎儿肠道中易化型葡萄糖转运蛋白（GLUT-1、GLUT-2和GLUT-5）的发育表达及定位。从胚胎和胎儿（妊娠第10至20天）、新生大鼠及成年大鼠（出生后16周）采集肠道。妊娠第10天时，GLUT-1在肠管中大量表达。从第10天至出生，GLUT-1持续表达但其表达逐渐降低。在成年大鼠中，未检测到GLUT-1。GLUT-2在第10至14天不表达，但在第16天，即在肠绒毛明显之前，开始在肠管腔中表达。在第18天，当肠绒毛正在发育时，GLUT-2定位于肠上皮细胞的基底外侧膜。在新生大鼠和成年大鼠肠道中也观察到类似的GLUT-2定位模式。GLUT-5的表达动力学与GLUT-2几乎相同。GLUT-5在第16天开始在肠管腔中表达。与GLUT-2不同，GLUT-5在第18天之后定位于肠绒毛的刷状缘膜。这些结果表明，葡萄糖转运蛋白家族各成员在胚胎和胎儿肠道中的表达及定位随妊娠期而变化。妊娠早期葡萄糖代谢可能需要GLUT-1，而妊娠晚期和围产期可能需要GLUT-2和GLUT-5。
