Okamoto K, Hirano H, Isohashi F
Department of Oncology, Osaka University Medical School, Japan.
Biochem Biophys Res Commun. 1993 Jun 30;193(3):848-54. doi: 10.1006/bbrc.1993.1703.
We previously found a new endogenous factor in rat liver cytosol, named ATP-stimulated glucocorticoid-receptor translocation promoter (ASTP), that increased the binding of activated glucocorticoid-receptor to nuclei in the presence of ATP. We have cloned and sequenced a cDNA (2989 bp) encoding the ASTP protein. An open reading frame of 524 amino acids encodes a protein with a molecular weight of 57 kDa. Amino-acid sequences of six peptides obtained by Edman degradation of various cleavage products of the purified ASTP were identified in the cDNA-derived sequence. Northern analysis of mRNA from rat liver revealed a major band of about 3.8 kb. Database searches show no homology to other known proteins from mammalian origin, however, the amino-acid sequence in the middle portion of ASTP protein shows 48% homology to glycerol kinase from Escherichia coli. The region of homology to other species may help to define sites of protein-protein interaction, as well as sites of possible interaction of ASTP with ATP.
我们之前在大鼠肝脏胞质溶胶中发现了一种新的内源性因子，命名为ATP刺激的糖皮质激素受体易位启动子（ASTP），在ATP存在的情况下，它能增加活化的糖皮质激素受体与细胞核的结合。我们已经克隆并测序了编码ASTP蛋白的cDNA（2989 bp）。一个524个氨基酸的开放阅读框编码一种分子量为57 kDa的蛋白质。通过对纯化的ASTP各种裂解产物进行埃德曼降解获得的六个肽段的氨基酸序列在cDNA推导的序列中得到了鉴定。对大鼠肝脏mRNA的Northern分析显示有一条约3.8 kb的主要条带。数据库搜索表明与其他已知的哺乳动物来源的蛋白质没有同源性，然而，ASTP蛋白中部的氨基酸序列与大肠杆菌的甘油激酶显示出48%的同源性。与其他物种的同源区域可能有助于确定蛋白质-蛋白质相互作用的位点，以及ASTP与ATP可能相互作用的位点。
