Girard P R, Nerem R M
Bioengineering Center, School of Mechanical Engineering, Georgia Institute of Technology, Atlanta 30332-0405.
Front Med Biol Eng. 1993;5(1):31-6.
Endothelial cells exposed to a fluid shear stress both in vivo and in vitro show an alignment with the direction of flow and an elongation of cells with a concomitant reorganization of their F-actin microfilament network. Specialized regions of the plasma membrane known as focal contacts are sites of transmembrane linkages between the actin microfilament bundles and the extracellular matrix (ECM) where cytoskeletal organization and, hence, cellular morphological changes may be modulated. Focal contact-associated proteins such as fibronectin receptors, vitronectin receptors and vinculin were shown in this study to play specific roles in the modulation of the cytoskeletal and cell shape changes. Data is also presented suggesting that protein kinase C is part of the cellular signaling system involved in shear stress-induced cytoskeletal reorganization.
在体内和体外暴露于流体剪切应力的内皮细胞会与流动方向对齐，细胞伸长，同时其F-肌动蛋白微丝网络会重新组织。质膜的特化区域称为黏着斑，是肌动蛋白微丝束与细胞外基质（ECM）之间跨膜连接的部位，细胞骨架组织以及细胞形态变化可能在此处受到调节。本研究表明，诸如纤连蛋白受体、玻连蛋白受体和纽蛋白等黏着斑相关蛋白在调节细胞骨架和细胞形状变化中发挥特定作用。还提供了数据表明蛋白激酶C是参与剪切应力诱导的细胞骨架重组的细胞信号系统的一部分。
