Lidbury B A, Aston R, Ramshaw I A, Cowden W B, Rathjen D A
Division of Cell Biology, John Curtin School of Medical Research, Australian National University, Canberra.
Lymphokine Cytokine Res. 1993 Apr;12(2):69-74.
This report describes the potentiation of the antiviral effects of human tumour necrosis factor-alpha (TNF-alpha) in vivo by specific antibodies. Complexes of TNF with either an anti-TNF monoclonal or polyclonal antibody at optimal doses induced a significantly greater antiviral (vaccinia virus) state in CBA/H mice than TNF alone. Furthermore, an antiviral synergy between murine gamma-interferon and TNF was found in vivo when the latter was in complex with enhancing antibody. Two other inbred mouse strains, C57/B6 and BALB/c, were also examined under these conditions. These antibodies greatly increase the binding of TNF to the surface of cells, which may account for the observed enhancement of TNF activity. Such antibodies may be of value clinically in viral diseases and cancer therapy where an increase in TNF activity, in the absence of side effects, would lead to more effective use of this cytokine in human therapy.
本报告描述了特异性抗体在体内增强人肿瘤坏死因子-α（TNF-α）抗病毒作用的情况。TNF与抗TNF单克隆或多克隆抗体以最佳剂量形成的复合物，在CBA/H小鼠中诱导出的抗病毒（痘苗病毒）状态比单独使用TNF时显著更强。此外，当TNF与增强抗体形成复合物时，在体内发现小鼠γ干扰素与TNF之间存在抗病毒协同作用。在这些条件下还检测了另外两个近交系小鼠品系C57/B6和BALB/c。这些抗体极大地增加了TNF与细胞表面的结合，这可能解释了所观察到的TNF活性增强现象。此类抗体在病毒疾病和癌症治疗中可能具有临床价值，在这些治疗中，在无副作用的情况下增加TNF活性将导致该细胞因子在人类治疗中得到更有效的应用。
