Voynikov T, Nikolova I, Yamanishi Y, Higashino H, Maeda M, Suzuki A
Department of Pharmacology, Kinki University School of Medicine, Osaka, Japan.
ASAIO J. 1993 Apr-Jun;39(2):141-4.
The effects of normothermic whole body rinse out (NWBRO) with FC-43 perfluorocarbon emulsion on the course of endotoxic shock were examined in male Wistar rats. These animals, conscious and unrestrained, were infused over 10 min with 60 mg/kg of E. coli endotoxin (LD100) and observed for 6 hr. The rats developed hypotension, plasma extravasation, metabolic acidosis, increase in serum GPT and GOT, and extensive hemorrhages in the small intestine. Another group of rats was subjected to blood substitution with FC-43 emulsion (artificial blood) 30 min after endotoxin infusion, until the hematocrit dropped to less than 3%, after which 6-9 ml of donor blood was exchange transfused with the emulsion. NWBRO caused a transitory rise in the GOT level, but more importantly it resulted in 98% endotoxin clearance, improved blood alkaline reserves, trimmed elevated GPT and GOT levels, and attenuated hemorrhage and epithelial necrosis in the small intestine.
在雄性Wistar大鼠中研究了用FC - 43全氟碳乳液进行常温全身冲洗（NWBRO）对内毒素休克病程的影响。这些清醒且未受束缚的动物在10分钟内输注60mg/kg的大肠杆菌内毒素（LD100），并观察6小时。大鼠出现低血压、血浆外渗、代谢性酸中毒、血清GPT和GOT升高以及小肠广泛出血。另一组大鼠在内毒素输注30分钟后用FC - 43乳液（人造血液）进行血液置换，直到血细胞比容降至3%以下，之后用6 - 9ml供体血液与乳液进行交换输血。NWBRO导致GOT水平短暂升高，但更重要的是，它实现了98%的内毒素清除，改善了血液碱储备，降低了升高的GPT和GOT水平，并减轻了小肠出血和上皮坏死。
