准确测定来自澳大利亚种群的自发性高血压大鼠肾素基因中第一个内含子缺失的大小。

Accurate determination of the size of the first intron deletion in the renin gene of SHR from an Australian colony.

Wang D Z, Gregorio-King C, Aykut L, Wilks A, Morgan T O, Di Nicolantonio R

Department of Physiology, University of Melbourne, Parkville, Victoria, Australia.

Clin Exp Pharmacol Physiol. 1993 May;20(5):343-5. doi: 10.1111/j.1440-1681.1993.tb01700.x.

Abstract

It has been reported that the first intron of the renin gene of the spontaneously hypertensive rat (SHR) is shorter than that of the Wistar-Kyoto (WKY) rat, suggesting a role for this difference in the development of hypertension. 2. The genealogy of some Australian SHR colonies has raised doubts about its similarity to the Japanese, American and European colonies. 3. The polymerase chain reaction (PCR) technique has been used with primers identical to the flanking region of the first intron of the renin gene to determine its length in SHR, WKY and Sprague-Dawley (SD) from an Australian colony. 4. The size of the first intron of the renin gene in SHR, WKY and SD was found to be approximately 3870, 4550 and 5000 base pairs, respectively, results which agree with those previously published. 5. It was concluded that the first intron deletion, and its size, has been conserved in an Australian SHR colony.

摘要

据报道，自发性高血压大鼠（SHR）肾素基因的第一个内含子比Wistar-Kyoto（WKY）大鼠的短，这表明这种差异在高血压的发展中起作用。2. 一些澳大利亚SHR群体的谱系引发了对其与日本、美国和欧洲群体相似性的质疑。3. 聚合酶链反应（PCR）技术已被用于与肾素基因第一个内含子侧翼区域相同的引物，以确定来自澳大利亚群体的SHR、WKY和Sprague-Dawley（SD）大鼠中该内含子的长度。4. 发现SHR、WKY和SD大鼠肾素基因第一个内含子的大小分别约为3870、4550和5000个碱基对，这一结果与先前发表的结果一致。5. 得出的结论是，第一个内含子的缺失及其大小在澳大利亚SHR群体中是保守的。