人脑肿瘤中DNA聚合酶α标记指数的测定。与体外溴脱氧尿苷（BUdR）标记指数及Ki-67的比较研究。
Measurement of labeling index of DNA polymerase alpha in human brain tumors. Comparative study with labeling indices of BUdR in vitro and Ki-67.
作者信息
Nishizaki T, Orita T, Kamiryo T, Akimura T, Kajiwara K, Ikeda N, Ohshita N, Ito H, Sasaki K
机构信息
Department of Neurosurgery, Yamaguchi University, School of Medicine, Japan.
出版信息
Clin Neuropathol. 1993 May-Jun;12(3):160-3.
Proliferative activity of 28 human brain tumors was estimated by simultaneous measurement of DNA polymerase alpha, Ki-67 and bromodeoxyuridine (BUdR) labeling indices on microscopic tissue preparations stained immunologically with monoclonal antibodies using a peroxidase technique. All the antigens were exclusively found in the nucleus. The labeling index of BUdR was lower than those of the other indicators. The values of the DNA polymerase alpha labeling index were almost the same as those of the Ki-67 labeling index. Simultaneous measurement of these parameters may provide more useful information on tumor cell growth kinetics than that of a single parameter.
通过使用过氧化物酶技术，在经单克隆抗体免疫染色的微观组织标本上同时测量DNA聚合酶α、Ki-67和溴脱氧尿苷（BUdR）标记指数，对28例人脑肿瘤的增殖活性进行了评估。所有抗原均仅存在于细胞核中。BUdR的标记指数低于其他指标。DNA聚合酶α标记指数的值与Ki-67标记指数的值几乎相同。与单一参数相比，同时测量这些参数可能会提供更多关于肿瘤细胞生长动力学的有用信息。
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