来自玉米黑粉菌的十字形DNA结合蛋白的纯化及特性

Purification and properties of a cruciform DNA binding protein from Ustilago maydis.

作者信息

Kotani H, Kmiec E B, Holloman W K

机构信息

Department of Pharmacology, Jefferson Cancer Institute, Thomas Jefferson University, Philadelphia, PA 19107.

出版信息

Chromosoma. 1993 May;102(5):348-54. doi: 10.1007/BF00661278.

DOI:10.1007/BF00661278

PMID:8325166

Abstract

A DNA binding protein with an M(r) of 11,000 was purified from Ustilago maydis. Its solubility in acid, amino acid composition, and mobility during gel electrophoresis are reminiscent of properties observed for the high mobility group nonhistone chromosomal proteins. The protein recognizes cruciform DNA made from oligonucleotides and also binds preferentially to a plasmid containing an extruded cruciform.

摘要

从玉米黑粉菌中纯化出一种分子量为11,000的DNA结合蛋白。它在酸中的溶解度、氨基酸组成以及凝胶电泳中的迁移率让人联想到高迁移率族非组蛋白染色体蛋白所具有的特性。该蛋白能识别由寡核苷酸形成的十字形DNA，并且还优先结合含有突出十字形结构的质粒。