Fletcher L D, Berger L C, Peel S A, Baric R S, Tidwell R R, Dykstra C C
Department of Pathology, School of Medicine, University of North Carolina, Chapel Hill 27599.
Gene. 1993 Jul 30;129(2):167-74. doi: 10.1016/0378-1119(93)90265-5.
Pneumocystis carinii pneumonia (PCP) is a leading cause of death among AIDS patients in the United States. Our analysis of P. carinii protein-coding genes has revealed a significant A + T codon bias. Polymerase chain reaction (PCR) was utilized to isolate and identify the genes encoding calmodulin, beta-tubulin, DNA polymerase II, and RNA polymerases I, II and III from P. carinii. Primer pairs were designed to incorporate P. carinii codon preference to known conserved protein regions from other organisms. This strategy should be useful for a large variety of P. carinii genes and assist in the comprehensive analysis of the genomic structure of this important pathogen.
卡氏肺孢子虫肺炎（PCP）是美国艾滋病患者的主要死因。我们对卡氏肺孢子虫蛋白质编码基因的分析显示出明显的A + T密码子偏好。利用聚合酶链反应（PCR）从卡氏肺孢子虫中分离并鉴定编码钙调蛋白、β-微管蛋白、DNA聚合酶II以及RNA聚合酶I、II和III的基因。设计引物对，使其结合卡氏肺孢子虫密码子偏好与来自其他生物的已知保守蛋白质区域。该策略对于大量卡氏肺孢子虫基因应是有用的，并有助于对这种重要病原体的基因组结构进行全面分析。
