在Xq28中由序列标签位点构建的1.5兆碱基酵母人工染色体重叠群，其中包括一个在克隆中不稳定的区域。

1.5-Mb YAC contig in Xq28 formatted with sequence-tagged sites and including a region unstable in the clones.

作者信息

Palmieri G, Romano G, Casamassimi A, D'Urso M, Little R D, Abidi F E, Schlessinger D, Lagerström M, Malmgren H, Steen-Bondeson M L

机构信息

International Institute of Genetics and Biophysics, Naples, Italy.

出版信息

Genomics. 1993 Jun;16(3):586-92. doi: 10.1006/geno.1993.1234.

DOI:10.1006/geno.1993.1234

PMID:8325630

Abstract

A contig of 20 yeast artificial clones (YACs) has been assembled across 1.5 Mb of Xq28 and formatted with nine previously reported probes and nine STSs developed from the sequence of probes and end fragments of YACs. YAC end fragments were obtained by subcloning, Alu-vector PCR, or primer-ligation PCR methods. Eighteen of the YACs were recovered from a library specific for Xq24-q28; two that fill a gap were obtained from a second library made from total human DNA. One region, containing probes pX78c and 2A1.1, was unstable in YACs, but it was possible to generate a self-consistent map of DNA over the entire contig. Overlaps were confirmed by Southern blot analyses of YAC DNAs, and pulsed-field gel electrophoresis confirmed the extent of the contig and identified at least four CpG islands in the region.

摘要

已经在Xq28的1.5兆碱基范围内组装了一个由20个酵母人工克隆（YAC）组成的重叠群，并使用9个先前报道的探针和9个从YAC探针和末端片段序列开发的序列标签位点（STS）进行了格式化。YAC末端片段通过亚克隆、Alu载体聚合酶链反应（PCR）或引物连接PCR方法获得。18个YAC是从针对Xq24 - q28的特异性文库中获得的；另外两个填补缺口的YAC是从由人类总DNA构建的第二个文库中获得的。一个包含探针pX78c和2A1.1的区域在YAC中不稳定，但有可能在整个重叠群上生成一个自洽的DNA图谱。通过对YAC DNA的Southern印迹分析证实了重叠情况，脉冲场凝胶电泳确定了重叠群的范围，并在该区域鉴定出至少四个CpG岛。