Eisinger D P, Serrero G
W. Alton Jones Cell Science Center, Lake Placid, New York 12946.
Genomics. 1993 Jun;16(3):638-44. doi: 10.1006/geno.1993.1241.
Adipose differentiation-related protein (ADRP) is a novel 50-kDa membrane-associated protein whose message levels are induced rapidly and maximally after triggering adipocyte differentiation. The gene encoding mouse ADRP has been isolated and characterized from four overlapping lambda phage clones. The gene spans 14 kb and contains 8 exons and 7 introns. Exons range in size from 50 to 696 bp and intron sizes range from 87 bp to 4.3 kb. Major and minor transcription initiation sites were determined 76 and 78 bp, respectively, upstream of the initiator methionine. A TATTTTA sequence is centered 30 bp upstream of the major transcription start site, and within the 5'-flanking region there are several putative transcription factor binding sites. ADRP has been mapped to chromosome 4, specifically between the b and Ifa loci. A second ADRP-like gene was isolated and partially characterized. This second locus is not expressed in 12 different mouse tissues and shares 87% sequence similarity to ADRP over exon and intron regions analyzed. Finally, this is the first reported genomic structure of ADRP.
脂肪分化相关蛋白（ADRP）是一种新的50 kDa膜相关蛋白，在触发脂肪细胞分化后，其信使水平迅速且最大程度地被诱导。从小鼠ADRP的四个重叠λ噬菌体克隆中分离并鉴定了编码该蛋白的基因。该基因跨度为14 kb，包含8个外显子和7个内含子。外显子大小从50到696 bp不等，内含子大小从87 bp到4.3 kb不等。分别在起始甲硫氨酸上游76和78 bp处确定了主要和次要转录起始位点。一个TATTTTA序列位于主要转录起始位点上游30 bp处，在5'侧翼区域内有几个假定的转录因子结合位点。ADRP已被定位到4号染色体，具体位于b和Ifa基因座之间。分离并部分鉴定了第二个类似ADRP的基因。该第二个基因座在12种不同的小鼠组织中不表达，在所分析的外显子和内含子区域与ADRP共享87%的序列相似性。最后，这是首次报道的ADRP基因组结构。
