Ross J R, Howell D N, Sanfilippo F P
Department of Pathology, Duke University, Durham, North Carolina.
Invest Ophthalmol Vis Sci. 1993 Jul;34(8):2469-76.
To characterize the fate of Lewis rat corneas transplanted to Hartley guinea pigs.
Full-thickness Lewis rat corneal buttons were grafted orthotopically to Hartley guinea pigs (xenografts), ACI rats (allografts), or Lewis rats (isografts). Two panels of recipients were presensitized with xenogeneic skin grafts or allogeneic skin grafts. Serum samples were collected pre- and post-transplant and analyzed by flow cytometry and indirect immunofluorescence.
Unlike vascularized xenografts that reject within 30 min, corneal xenografts had a mean survival time of 8 days. Presensitization with guinea pig skin grafts increased recipient IgM and IgG xenoantibody levels, as measured by flow cytometry on guinea pig hematopoietic cells, and significantly accelerated corneal xenograft rejection with a mean survival time of 5 days. Presensitization with allogeneic ACI skin grafts had no effect on xenoantibody levels or xenogeneic corneal graft survival. Guinea pig corneas stained by indirect immunofluorescence with normal rat serum exhibited low (1+) but significant binding of IgG and IgM, primarily on epithelium and stroma. Serum from Lewis rats that rejected a corneal xenograft had elevated IgG and IgM xenoantibodies that reacted strongly (4+) with guinea pig cornea and heart.
In the discordant guinea pig-to-rat species combination, donor corneas express xenoantigens; rejection of corneal xenografts stimulates IgM and IgG xenoantibody production; sensitization to xenoantigens can accelerate corneal xenograft rejection; and discordant corneal xenografts, unlike vascularized organs, are not hyperacutely rejected.
描述移植到哈特利豚鼠体内的刘易斯大鼠角膜的转归。
将刘易斯大鼠全层角膜植片原位移植到哈特利豚鼠（异种移植）、ACI大鼠（同种异体移植）或刘易斯大鼠（同基因移植）体内。两组受体分别用异种皮肤移植或同种异体皮肤移植进行预致敏。在移植前后收集血清样本，通过流式细胞术和间接免疫荧光进行分析。
与在30分钟内排斥的血管化异种移植不同，角膜异种移植的平均存活时间为8天。用豚鼠皮肤移植进行预致敏可提高受体IgM和IgG异种抗体水平，通过对豚鼠造血细胞进行流式细胞术检测可知，这显著加速了角膜异种移植排斥，平均存活时间为5天。用同种异体ACI皮肤移植进行预致敏对异种抗体水平或异种角膜移植存活没有影响。用正常大鼠血清通过间接免疫荧光对豚鼠角膜染色显示，IgG和IgM有低水平（1+）但显著的结合，主要在上皮和基质。排斥角膜异种移植的刘易斯大鼠血清中IgG和IgM异种抗体升高，与豚鼠角膜和心脏有强烈反应（4+）。
在豚鼠到大鼠这种不匹配的物种组合中，供体角膜表达异种抗原；角膜异种移植排斥刺激IgM和IgG异种抗体产生；对异种抗原致敏可加速角膜异种移植排斥；与血管化器官不同，不匹配的角膜异种移植不会发生超急性排斥。
