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高温在体外和体内均可诱导大鼠心脏细胞中转化生长因子-β的表达。

Hyperthermia induces expression of transforming growth factor-beta s in rat cardiac cells in vitro and in vivo.

作者信息

Flanders K C, Winokur T S, Holder M G, Sporn M B

机构信息

Laboratory of Chemoprevention, National Cancer Institute, Bethesda, Maryland 20892.

出版信息

J Clin Invest. 1993 Jul;92(1):404-10. doi: 10.1172/JCI116581.

DOI:10.1172/JCI116581
PMID:8326008
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC293625/
Abstract

Hyperthermia causes changes in expression of TGF-beta mRNA and protein in cultured cardiac cells, as well as in the heart in vivo. 12 h after hyperthermia, primary cultures of neonatal rat cardiomyocytes show a two- to threefold decreased expression of TGF-beta mRNAs which returns to control levels by 48 h after heat shock. In cultures of cardiac fibroblasts, expression of TGF-beta mRNAs increases 5-25-fold, 12-48 h after heat shock, while fetal bovine heart endothelial cells show little change in TGF-beta expression after hyperthermia. In each case, mRNAs for TGF-beta s 1, 2, and 3 are regulated similarly. Hearts isolated from rats exposed to hyperthermia show an initial 20-fold decrease in TGF-beta 1 and 3 mRNA levels which return to control levels by 24 h and subsequently are elevated two- to threefold above normal 48-72 h after heat shock; there is little change in TGF-beta 2 mRNA. Expression of immunoreactive TGF-beta 1 and 3 protein, localized intracellularly in myocytes, follows the same pattern as the mRNA expression. By 72 h, some myocytes show hyperstaining for TGF-beta 1. Staining for extracellular TGF-beta 1/3 exhibits the opposite time course, being most intense 3-6 h after heat shock and returning to control levels by 48 h. The increase in TGF-beta s after hyperthermia could play a role in mediating the reported cardioprotective effects of heat shock.

摘要

热疗可导致培养的心脏细胞以及体内心脏中转化生长因子β（TGF-β）mRNA和蛋白质表达的变化。热疗12小时后，新生大鼠心肌细胞原代培养物中TGF-β mRNA的表达下降了2至3倍，热休克后48小时恢复到对照水平。在心脏成纤维细胞培养物中，热休克后12至48小时，TGF-β mRNA的表达增加了5至25倍，而胎牛心脏内皮细胞在热疗后TGF-β表达几乎没有变化。在每种情况下，TGF-β 1、2和3的mRNA调控方式相似。从暴露于热疗的大鼠分离出的心脏显示，TGF-β 1和3 mRNA水平最初下降20倍，24小时后恢复到对照水平，随后在热休克后48至72小时比正常水平升高2至3倍；TGF-β 2 mRNA几乎没有变化。免疫反应性TGF-β 1和3蛋白在心肌细胞内的表达模式与mRNA表达相同。到72小时时，一些心肌细胞显示TGF-β 1染色过深。细胞外TGF-β 1/3的染色呈现相反的时间进程，在热休克后3至6小时最为强烈，48小时后恢复到对照水平。热疗后TGF-β的增加可能在介导热休克所报道的心脏保护作用中发挥作用。

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