Middlebrook J L, Aronow L
Endocrinology. 1977 Feb;100(2):271-82. doi: 10.1210/endo-100-2-271.
Glucocorticoid-bound receptor macromolecules were prepared in three forms from mouse fibroblast cells: the cytosol receptor, the nuclear extractable receptor, and the nuclear residual form obtained by DNase digestion of chromatin samples. These receptor complexes were studied with respect to gel filtration properties, sedimentation velocities in various salt concentrations, partial specific volumes, isoelectric points, and thermal stability properties. The results indicate that the three forms of the receptor differ in their molecular properties, and nuclear translocation and binding ofthe receptor complex is associated with conformational and physical changes consistent with a reduction in apparent molecular weight.
胞质溶胶受体、可从细胞核中提取的受体以及通过对染色质样品进行脱氧核糖核酸酶消化获得的细胞核残余形式。对这些受体复合物进行了凝胶过滤特性、在各种盐浓度下的沉降速度、偏比容、等电点和热稳定性特性方面的研究。结果表明，受体的三种形式在分子特性上有所不同，并且受体复合物的核转位和结合与构象和物理变化相关，这些变化与表观分子量的降低一致。
