Tager H S, Hohenboken M, Markese J
Endocrinology. 1977 Feb;100(2):367-72. doi: 10.1210/endo-100-2-367.
Antibody titers in rabbits immunized with glucagon conjugated to albumin using difluorodinitrobenzene rose rapidly. Under conditions of immunoassay, less than 2 nl of serum from two of four animals and approximately 4 nl from the other two was required to bind 50% of the 10 pg of [125I]iodoglucagon 100 days after immunization. The dissociation constants of the two higher titer antisera for glucagon were approximately 1 x 10(-10)M, and their binding capacities for the hormone, about 50 mug/ml. Competitive binding assays showed that neither of these antisera cross-reacts with the glucagon-like, immunoreactive peptides extracted from intestine to greater than 2.5%. In contrast, hens produced antisera which were reactive with the intestinal material and which bound only 0.3 mug of glucagon per ml. There were no consistent differences, however, in the abilities of specific and non-specific antisera to react with selected fragments of pancreatic glucagon.
用二氟二硝基苯将胰高血糖素与白蛋白偶联后免疫的兔子，其抗体效价迅速上升。在免疫测定条件下，免疫后100天，四只动物中有两只动物血清用量不到2 nl，另外两只动物约4 nl，就能结合10 pg [125I]碘胰高血糖素的50%。两种高效价抗血清对胰高血糖素的解离常数约为1×10⁻¹⁰M，它们对该激素的结合能力约为50 μg/ml。竞争性结合试验表明，这两种抗血清与从肠道提取的胰高血糖素样免疫反应性肽的交叉反应均不超过2.5%。相比之下，母鸡产生的抗血清与肠道物质有反应，每毫升仅能结合0.3 μg胰高血糖素。然而，特异性和非特异性抗血清与胰腺胰高血糖素选定片段反应的能力没有一致的差异。
