Moran C G, McGrory B J, Roorda J, Wood M B
Department of Orthopedics, Mayo Graduate School of Medicine, Rochester, MN 55905.
J Orthop Res. 1993 May;11(3):429-37. doi: 10.1002/jor.1100110316.
A vascularized canine tibial allograft was used to study the alpha-adrenoreceptor subtypes of the microcirculation of bone. Bone transplantation was performed on six dogs, and the bone blood flow was estimated with the use of serial injections of radiolabeled 15 microns microspheres. After microvascular anastomosis, the mean cortical blood flow in the allografted tibia was 3.6 +/- 2.1 ml/100 g/min. Alpha 1-adrenoreceptor blockade, with prazosin, caused an increase in normalized blood flow in five allografts and a slight decrease in one allograft. The mean increase was 32 +/- 48%, but this was not statistically significant (p < 0.2). The addition of an alpha 2-adrenoreceptor blockade, with rauwolszin, resulted in a significant increase (132 +/- 88%) in normalized blood flow in all allografts (p < 0.02). This ex vivo experiment confirms that both alpha 1 and alpha 2 adrenergic mechanisms play a role in controlling bone blood flow in centrally denervated allografts.
采用带血管的犬胫骨同种异体移植来研究骨微循环的α-肾上腺素能受体亚型。对6只犬进行骨移植，通过连续注射放射性标记的15微米微球来估计骨血流量。微血管吻合后，同种异体移植胫骨的平均皮质血流量为3.6±2.1毫升/100克/分钟。用哌唑嗪进行α1-肾上腺素能受体阻滞，导致5个同种异体移植的标准化血流量增加，1个同种异体移植的血流量略有下降。平均增加32±48%，但无统计学意义（p<0.2）。加入用萝芙木碱进行的α2-肾上腺素能受体阻滞，导致所有同种异体移植的标准化血流量显著增加（132±88%）（p<0.02）。这项体外实验证实，α1和α2肾上腺素能机制在控制去神经同种异体移植骨的骨血流量中均起作用。
