维生素D结合蛋白的结合与细胞分化有关吗？

Is binding of vitamin D binding protein related to cell differentiation?

作者信息

Petrini M, Valentini P, Allegrini A, Sabbatini A, Testi R, Ambrogi F, Arnaud P, Galbraith R M

机构信息

Haematology Unit, University of Pisa, Italy.

出版信息

Leuk Res. 1993 Jul;17(7):561-5. doi: 10.1016/0145-2126(93)90085-y.

DOI:10.1016/0145-2126(93)90085-y

PMID:8326737

Abstract

Vitamin D binding protein (Gc) is present on the surface of several blood cells and may interfere with the activity of 1,25(OH)2D3. It has previously been reported that Gc may bind to the U-937 line which is known to differentiate upon exposition to 1,25(OH)2D3. In the present paper, we evaluate the expression of Gc on the surface of U-937 and HL-60 lines. Both cell lines did not express Gc on their surface but U-937 cells were able to bind human purified Gc added to the medium whereas HL-60 were not. After culturing with 1,25(OH)2D3, HL-60 became able to bind Gc. This property seems to be related to the monocytic differentiation induced by 1,25(OH)2D3. Conversely, when present together, 1,25(OH)2D3 reduces binding on U-937.

摘要

维生素D结合蛋白（Gc）存在于多种血细胞表面，可能会干扰1,25(OH)₂D₃的活性。此前有报道称，Gc可能与U - 937细胞系结合，已知该细胞系在暴露于1,25(OH)₂D₃时会发生分化。在本文中，我们评估了Gc在U - 937和HL - 60细胞系表面的表达情况。这两种细胞系表面均未表达Gc，但U - 937细胞能够结合添加到培养基中的人纯化Gc，而HL - 60细胞则不能。用1,25(OH)₂D₃培养后，HL - 60细胞变得能够结合Gc。这种特性似乎与1,25(OH)₂D₃诱导的单核细胞分化有关。相反，当同时存在时，1,25(OH)₂D₃会减少其与U - 937细胞的结合。