Murphy M J, MacPherson G G, Camiscoli J F, Gordon A S
Exp Hematol. 1977 Jan;5(1):41-50.
Spontaneously flowing fistulae were established in the efferent lymphatics of popliteal, prescapular and prefemoral nodes and lumbar trunk or in the afferent lymphatics draining the kidney and liver of sheep. Lymph was collected from these sites over various time intervals and assayed for erythropoietin (Ep) content. The objective of the study was to establish the anatomic site(s) of Ep production. Normal lymph did not contain detectable titers of Ep, nor did renal lymph or blood plasma from a sheep systematically treated with cobaltous chloride. Renal lymph did contain measurable levels of Ep following renal artery constriction, unilateral hydronephrosis or phenylhydrazine-induced hemolytic anemia. Phenylhydrazine treatment also produced elevated Ep levels in lymph from the liver but not in lymph efferent from either popliteal or prescapular nodes. These results indicate that Ep is generated primarily in the kidney and that the liver may be an extrarenal source of the hormone. The surgical techniques used in this study offer distinct advantages in examining the composition and physiology of lymph in sheep.
在绵羊腘淋巴结、肩胛前淋巴结、股前淋巴结及腰干的输出淋巴管或引流肾脏和肝脏的输入淋巴管中建立了自然流动的瘘管。在不同时间间隔从这些部位收集淋巴液，并检测促红细胞生成素（Ep）含量。该研究的目的是确定Ep产生的解剖部位。正常淋巴液中未检测到可检测水平的Ep，用氯化钴系统治疗的绵羊的肾淋巴液或血浆中也未检测到。在肾动脉狭窄、单侧肾积水或苯肼诱导的溶血性贫血后，肾淋巴液中确实含有可测量水平的Ep。苯肼治疗还使肝脏淋巴液中的Ep水平升高，但腘淋巴结或肩胛前淋巴结的输出淋巴液中的Ep水平未升高。这些结果表明，Ep主要在肾脏中产生，肝脏可能是该激素的肾外来源。本研究中使用的手术技术在检查绵羊淋巴液的成分和生理学方面具有明显优势。
