Tamaru T, Trigun S K, Okada M, Nakagawa H
Division of Protein Metabolism, Institute for Protein Research, Osaka University, Japan.
Neurosci Lett. 1993 Apr 30;153(2):169-72. doi: 10.1016/0304-3940(93)90314-b.
The multiple cyclins and their catalytic subunit, cdc2-related kinase, play essential roles in the eucaryotic cell cycle. To examine their potential roles in highly differentiated nervous system, we determined their activity and localization in rat brain. p13suc1 associated histone H1 kinase activity was dramatically increased at the onset of brain maturation. The increased kinase activity was coprecipitated with cyclin D1, a type of G1 cyclin, which also increased with brain maturation. Immunohistochemical analysis demonstrated that anti-cyclin D1-like immunoreactivity was exclusively localized to neurons. From these findings, it is suggested that a type of cdc2-related kinase regulated by cyclin D1 might function in neurons independent of cell cycle progression.
多种细胞周期蛋白及其催化亚基，即与细胞分裂周期蛋白2相关的激酶，在真核细胞周期中发挥着重要作用。为了研究它们在高度分化的神经系统中的潜在作用，我们测定了它们在大鼠脑中的活性和定位。与p13suc1相关的组蛋白H1激酶活性在脑成熟开始时显著增加。增加的激酶活性与细胞周期蛋白D1（一种G1期细胞周期蛋白）共沉淀，细胞周期蛋白D1也随着脑成熟而增加。免疫组织化学分析表明，抗细胞周期蛋白D1样免疫反应性仅定位于神经元。从这些发现可以推测，一种受细胞周期蛋白D1调节的与细胞分裂周期蛋白2相关的激酶可能在神经元中发挥作用，而与细胞周期进程无关。
