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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Krucher N A, Meijer L, Roberts M H

Department of Chemistry, Clarkson University, Potsdam, New York 13699-5805, USA.

Cell Mol Neurobiol. 1997 Oct;17(5):495-507. doi: 10.1023/a:1026358821640.

In this study, we determined the effects of the cyclin-dependent kinase (cdk) inhibitors, olomoucine and roscovitine, on the circadian rhythm of optic nerve impulse activity recorded from the eye of the marine snail Bulla gouldiana. 2. We found that olomoucine lengthened period and altered circadian phase in a dose-dependent manner without appreciably affecting gene transcription or translation. We also found that the more specific cdk inhibitor, roscovitine, was approximately 10-fold more effective in lengthening circadian period, while the inactive analogue, iso-olomoucine, was ineffective. 3. The current results, along with previous results from our laboratory, are consistent with the hypothesis that the biochemical mechanism responsible for generating the ocular circadian rhythm in B. gouldiana is related to the biochemical mechanism that regulates the eukaryotic cell division cycle, i.e., by modulation of the activity of protein kinases belonging to the cdk family.

在本研究中，我们测定了细胞周期蛋白依赖性激酶（cdk）抑制剂olomoucine和roscovitine对从海蜗牛Bulla gouldiana眼睛记录到的视神经冲动活动昼夜节律的影响。2. 我们发现olomoucine以剂量依赖的方式延长了周期并改变了昼夜节律相位，而对基因转录或翻译没有明显影响。我们还发现，更具特异性的cdk抑制剂roscovitine在延长昼夜周期方面的效果约为其10倍，而无活性类似物异olomoucine则无效。3. 目前的结果与我们实验室之前的结果一致，支持这样一种假说，即负责产生B. gouldiana眼部昼夜节律的生化机制与调节真核细胞分裂周期的生化机制有关，即通过调节属于cdk家族的蛋白激酶的活性。

Krucher N A, Meijer L, Roberts M H

Department of Chemistry, Clarkson University, Potsdam, New York 13699-5805, USA.

Cell Mol Neurobiol. 1997 Oct;17(5):495-507. doi: 10.1023/a:1026358821640.

In this study, we determined the effects of the cyclin-dependent kinase (cdk) inhibitors, olomoucine and roscovitine, on the circadian rhythm of optic nerve impulse activity recorded from the eye of the marine snail Bulla gouldiana. 2. We found that olomoucine lengthened period and altered circadian phase in a dose-dependent manner without appreciably affecting gene transcription or translation. We also found that the more specific cdk inhibitor, roscovitine, was approximately 10-fold more effective in lengthening circadian period, while the inactive analogue, iso-olomoucine, was ineffective. 3. The current results, along with previous results from our laboratory, are consistent with the hypothesis that the biochemical mechanism responsible for generating the ocular circadian rhythm in B. gouldiana is related to the biochemical mechanism that regulates the eukaryotic cell division cycle, i.e., by modulation of the activity of protein kinases belonging to the cdk family.

在本研究中，我们测定了细胞周期蛋白依赖性激酶（cdk）抑制剂olomoucine和roscovitine对从海蜗牛Bulla gouldiana眼睛记录到的视神经冲动活动昼夜节律的影响。2. 我们发现olomoucine以剂量依赖的方式延长了周期并改变了昼夜节律相位，而对基因转录或翻译没有明显影响。我们还发现，更具特异性的cdk抑制剂roscovitine在延长昼夜周期方面的效果约为其10倍，而无活性类似物异olomoucine则无效。3. 目前的结果与我们实验室之前的结果一致，支持这样一种假说，即负责产生B. gouldiana眼部昼夜节律的生化机制与调节真核细胞分裂周期的生化机制有关，即通过调节属于cdk家族的蛋白激酶的活性。