Legendre J M, Bergot A, Turzo A, Morin P P
UBO-UFR de Médecine, Laboratoire de Biophysique, Brest, France.
Pathol Biol (Paris). 1993 Feb;41(2):169-71.
Isoelectrofocusing analysis of the murine monoclonal antibody OC 125 (IgG1) revealed four bands at pI (6.6-7.2). After pepsin digestion, f(ab')2 pIs were increased by 0.6 pH units and two additional bands were visible. DTPA conjugation decreased pI (by up to -2 units) and increased heterogeneity (10 bands or more). With In3+ at saturation level, the conjugate was slightly modified. Autoradiography revealed pI heterogeneity of OC 125-f(ab')2-DTPA-In-111. These data show that isoelectrofocusing enables accurate monitoring of monoclonal antibody changes during In-111 labeling.
对鼠单克隆抗体OC 125（IgG1）进行等电聚焦分析，结果显示在pI（6.6 - 7.2）处有四条带。经胃蛋白酶消化后，f(ab')2的pI增加了0.6个pH单位，并且可见另外两条带。二乙三胺五乙酸（DTPA）偶联使pI降低（最多降低2个单位）并增加了异质性（10条带或更多）。当铟离子（In3+）达到饱和水平时，偶联物略有改变。放射自显影显示了OC 125 - f(ab')2 - DTPA - 铟 - 111的pI异质性。这些数据表明，等电聚焦能够准确监测111铟标记过程中单克隆抗体的变化。
