[电子显微镜下mRNA的检测：包埋前生物素标记寡核苷酸的原位杂交]
[Detection of mRNA in electronic microscopy: in situ hybridization of a biotin-labeled oligonucleotide prior to embedding].
作者信息
Trembleau A
机构信息
Laboratoire de Neurobiologie des Signaux Intercellulaires, CNRS URA 1488, Université P. et M. Curie, Paris, France.
出版信息
Pathol Biol (Paris). 1993 Feb;41(2):194-7.
PMID:8327271
Abstract
A technique for mRNA detection by ultrastructural in situ hybridization is reported. Vibratome sections are hybridized with a biotinylated oligonucleotide probe which is detected using an enhanced avidin-peroxidase technique. Sections are then osmicated and embedded in epoxy resin. This rapid, sensitive technique achieved ultrastructural detection of vasopressin mRNA in magnocellular neurons of rat hypothalamus.
摘要
报道了一种通过超微结构原位杂交检测mRNA的技术。振动切片机切片与生物素化的寡核苷酸探针杂交，该探针使用增强抗生物素蛋白-过氧化物酶技术进行检测。然后对切片进行锇酸处理并包埋在环氧树脂中。这种快速、灵敏的技术实现了对大鼠下丘脑大细胞神经元中血管加压素mRNA的超微结构检测。
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