Turner T T, Hartmann P K, Howards S S
Fertil Steril. 1977 Feb;28(2):191-4. doi: 10.1016/s0015-0282(16)42382-4.
In vivo samples of epididymal fluids were obtained through the use of micropuncture techniques. Microsamples from four areas of the rat epididymis were analyzed for Na+ and K+ concentrations and for sperm density. Na+ values declined significantly from caput to corpus epididymidis (P less than 0.01), while K+ and sperm concentrations increased significantly (P less than 0.01). A large water loss from the epididymal lumen was calculated, as well as net losses of both cations. Water losses may be explained on the basis of an active Na+ pump; however, the effect of the absolute values of epididymal Na+ and K+ concentrations on sperm motility and fertility remains unresolved.
通过微穿刺技术获取附睾液的体内样本。对大鼠附睾四个区域的微量样本进行了钠(Na⁺)和钾(K⁺)浓度以及精子密度分析。从附睾头到附睾体,Na⁺值显著下降(P < 0.01),而K⁺和精子浓度显著增加(P < 0.01)。计算得出附睾管腔有大量水分流失以及两种阳离子的净流失。水分流失可能基于活跃的Na⁺泵来解释;然而,附睾中Na⁺和K⁺浓度的绝对值对精子活力和生育能力的影响仍未解决。