Hoving J, Wilson J H, Valkema A J, Woldring M G
Gastroenterology. 1977 Mar;72(3):406-12.
The simultaneous use of 131I-triolein and 75Se-triether, a nonabsorbable marker, in a single oral dose to estimate fat absorption from incomplete fecal collections was tested in rats with and without induced steatorrhea. The results in normal rats showed that analysis of single stool samples allows a valid estimate of fat absorption as defined by the balance study based on the total fecal recovery of 131I. However, in the few normal rats with poor absorption, the absorption values from successively excreted stools showed a tendency to increase. Similar findings were obtained from biliary fistula rats with marked steatorrhea. No evidence of different intestinal transit rates of test fat and marker was observed in bile-diverted rats, thus suggesting that the observed inconsistency in fat absorption values from different stool specimens reflects intraprandial differences in the absorption of fat. Studies on rats subjected to intestinal ischemia do not support the suggestion of others that separation of fat and triether will occur if a mucosal defect is involved as the cause of fat malabsorption. It is concluded that this dual isotope technique may be of value in the clinical estimation of fat absorption, as it offers important technical advantages over conventional fat balance studies.
在有或无诱发性脂肪泻的大鼠中，测试了单次口服剂量的131I-三油酸甘油酯和一种不可吸收的标记物75Se-三醚，以从不完全粪便收集中估计脂肪吸收情况。正常大鼠的结果表明，基于131I的总粪便回收率，对单个粪便样本进行分析能够对平衡研究所定义的脂肪吸收情况做出有效的估计。然而，在少数吸收不良的正常大鼠中，相继排出的粪便的吸收值呈上升趋势。从有明显脂肪泻的胆瘘大鼠中也得到了类似的结果。在胆汁分流的大鼠中，未观察到测试脂肪和标记物在肠道转运速率上的差异，因此表明，从不同粪便样本中观察到的脂肪吸收值的不一致反映了餐内脂肪吸收的差异。对肠缺血大鼠的研究并不支持其他人提出的观点，即如果黏膜缺陷是脂肪吸收不良的原因，脂肪和三醚会发生分离。得出的结论是，这种双同位素技术在临床评估脂肪吸收方面可能具有价值，因为与传统的脂肪平衡研究相比，它具有重要的技术优势。
