Filip V, Kleinová M
Chemisch-technologische Hochschule, Institut für Milch- und Fettechnologie, Praha.
Z Lebensm Unters Forsch. 1993 Jun;196(6):532-5. doi: 10.1007/BF01201333.
The determination of monoglycerides, diglycerides and triglycerides in the normal phase system: Separon SGX-CN (silica gel with phase-bonded cyanoethyl) and mobile phase hexane-isopropanol-formic acid by HPLC with refractometric detection, is described. It was found that the separation was according to the molecular mass of glycerides, i.e. in groups. This was demonstrated with palmitic, oleic and linoleic acids. The diglycerides and especially monoglycerides of olive and sunflower oils were partly separated according to the ECN1 values of the glycerides. The response factors related to triglycerides were estimated for the glycerides of the fatty acids and oils mentioned above. The value of response factors depends on the number of double bonds of the fatty acids.
正相体系中甘油单酯、甘油二酯和甘油三酯的测定：描述了采用带示差折光检测的高效液相色谱法，使用Separon SGX-CN（键合氰基乙硅烷基的硅胶）和流动相己烷-异丙醇-甲酸来测定。发现分离是根据甘油酯的分子量进行的，即按类别分离。这通过棕榈酸、油酸和亚油酸得到了证明。橄榄油和向日葵油的甘油二酯，尤其是甘油单酯，部分是根据甘油酯的ECN1值进行分离的。对上述脂肪酸和油类的甘油酯，估算了与甘油三酯相关的响应因子。响应因子的值取决于脂肪酸双键的数量。
