[Determination of mono-, di- and triglycerides using HPLC].

The determination of monoglycerides, diglycerides and triglycerides in the normal phase system: Separon SGX-CN (silica gel with phase-bonded cyanoethyl) and mobile phase hexane-isopropanol-formic acid by HPLC with refractometric detection, is described. It was found that the separation was according to the molecular mass of glycerides, i.e. in groups. This was demonstrated with palmitic, oleic and linoleic acids. The diglycerides and especially monoglycerides of olive and sunflower oils were partly separated according to the ECN1 values of the glycerides. The response factors related to triglycerides were estimated for the glycerides of the fatty acids and oils mentioned above. The value of response factors depends on the number of double bonds of the fatty acids.