Hu Y Z, He H, Lai Y H, Ni K Y
China Pharmaceutical University, Nanjing.
Yao Xue Xue Bao. 1993;28(1):50-6.
A new experimental design method named the step climbing method (SCM), as well as a new global criterion (NRC), are proposed for the selectivity optimization of thin layer chromatography. The method offers chromatographers a convenient means to decide the optimum developer composition for thin layer chromatography. The principle of the method is described, and examples of applications in pharmaceutical analysis are given to certify the feasibility of the method. Clearly, it may also be used for the selectivity optimization in high performance liquid chromatography.
提出了一种名为逐步攀升法（SCM）的新实验设计方法以及一种新的全局判据（NRC），用于薄层色谱的选择性优化。该方法为色谱工作者提供了一种便捷的手段来确定薄层色谱的最佳展开剂组成。阐述了该方法的原理，并给出了在药物分析中的应用实例以证明该方法的可行性。显然，它也可用于高效液相色谱的选择性优化。
