Cheng Y H, Liao G T, Hou S X, Li L, Zhang M
School of Pharmacy, West China University of Medical Sciences, Chengdu.
Yao Xue Xue Bao. 1993;28(1):68-74.
In this paper 6 factors and 12 levels of each variable were selected by Uniform Design Method and computer for preparing albumin microspheres with emulsion-chemical cross-linking. An optimal procedure for preparing albumin microspheres was established and the mean diameter of albumin microspheres is 0.41-0.47 micron. Albumin was labelled with 125I-isotope and 125I-albumin microspheres were prepared according to the optimal procedure. The suspension of 125I-albumin microspheres with 0.1% Tween-80 saline was injected via mice tail vein. The animals were sequentially killed and the radioactivity of blood, spleen, liver, kidney, stomach, heart, lung, thyroid and brain were measured. The results showed that albumin microspheres were accumulated mainly in the liver, about 68% of the injected dose at the peak concentration. The pharmacokinetics of albumin microspheres in liver was also studied and two-compartmental model can be used to describe the regulation.
本文采用均匀设计法并借助计算机，选取了6个因素及每个变量的12个水平，用于通过乳化-化学交联法制备白蛋白微球。建立了制备白蛋白微球的优化工艺，白蛋白微球的平均直径为0.41 - 0.47微米。用125I-同位素标记白蛋白，并按照优化工艺制备125I-白蛋白微球。将含0.1%吐温-80生理盐水的125I-白蛋白微球混悬液经小鼠尾静脉注射。依次处死动物，测定血液、脾脏、肝脏、肾脏、胃、心脏、肺、甲状腺和脑的放射性。结果表明，白蛋白微球主要蓄积在肝脏，峰值浓度时约为注射剂量的68%。还研究了白蛋白微球在肝脏中的药代动力学，可用二室模型描述其规律。
