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柱分配色谱预分离后用褶合曲线法测定复方茶碱片中六种主要成分

[Determination of six main components in compound theophylline tablet by convolution curve method after prior separation by column partition chromatography].

作者信息

Zhang S Y, Wang G F, Wu Y T

机构信息

First Military Medical University, Guangzhou.

出版信息

Yao Xue Xue Bao. 1993;28(2):126-9.

PMID:8328281
Abstract

On a partition chromatographic column in which the support is Kieselguhr and the stationary phase is sulfuric acid solution (2 mol/L), three components of compound theophylline tablet were simultaneously eluted by chloroform and three other components were simultaneously eluted by ammonia-saturated chloroform. The two mixtures were determined by computer-aided convolution curve method separately. The corresponding average recovery and relative standard deviation of the six components were as follows: 101.6, 1.46% for caffeine; 99.7, 0.10% for phenacetin; 100.9, 1.31% for phenobarbitone; 100.2, 0.81% for theophylline; 99.9, 0.81% for theobromine and 100.8, 0.48% for aminopyrine.

摘要

在以硅藻土为载体、硫酸溶液(2mol/L)为固定相的分配色谱柱上,复方茶碱片中的三种成分被氯仿同时洗脱,另外三种成分被氨饱和氯仿同时洗脱。这两种混合物分别采用计算机辅助褶合曲线法测定。六种成分相应的平均回收率和相对标准偏差如下:咖啡因,101.6,1.46%;非那西丁,99.7,0.10%;苯巴比妥,100.9,1.31%;茶碱,100.2,0.81%;可可碱,99.9,0.81%;氨基比林,100.8,0.48%。

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