Sato T, Takei K, Nonoyama T, Miyauchi T, Goto K, Hommura S
Department of Ophthalmology, University Hospital of Tsukuba, Japan.
Nippon Ganka Gakkai Zasshi. 1993 Jun;97(6):683-9.
Endothelin-1 (ET-1) is an endothelium-derived potent vasoconstrictor peptide which is synthesized in retinal vessels. In order to investigate its effect on retinal blood vessels, we injected ET-1 intravitreally (10(-12), 3 x 10(-12), 10(-11), 3 x 10(-11), 10(-10) mole) and examined the change in diameters of retinal arteries in rabbits by fundus photography. After injection of a high dose (10(-10) mole) of ET-1, fluorescein angiography was used to investigate whether complete occlusions of retinal blood vessels occurred or not. 3 x 10(-12) mole ET-1 induced contraction of retinal arteries and the contraction of those induced by 10(-10) mole ET-1 showed complete occlusion. A medium dose (10(-11) mole) of ET-1 caused repeated vasospasms and segmental vasoconstrictions of retinal blood vessels. Fluorescein angiograms revealed that retinal blood flow was interrupted transiently by injection of a high dose (10(-10) mole) of ET-1. These findings suggested that intravitreal injection of ET-1 could provide a new model of experimental transient occlusion of retinal vessels in rabbits.
内皮素-1（ET-1）是一种由内皮细胞产生的强效血管收缩肽，在视网膜血管中合成。为了研究其对视网膜血管的作用，我们经玻璃体注射ET-1（10^(-12)、3×10^(-12)、10^(-11)、3×10^(-11)、10^(-10)摩尔），并通过眼底摄影检查兔视网膜动脉直径的变化。注射高剂量（10^(-10)摩尔）的ET-1后，使用荧光素血管造影术研究视网膜血管是否发生完全闭塞。3×10^(-12)摩尔的ET-1可引起视网膜动脉收缩，而10^(-10)摩尔的ET-1所引起的收缩则显示完全闭塞。中等剂量（10^(-11)摩尔）的ET-1会导致视网膜血管反复发生血管痉挛和节段性血管收缩。荧光素血管造影显示，注射高剂量（10^(-10)摩尔）的ET-1会使视网膜血流短暂中断。这些发现表明，经玻璃体注射ET-1可为兔视网膜血管实验性短暂闭塞提供一种新模型。
