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1
The isolation and identification of 6-hydroxycyclohepta-1,4-dione as a novel intermediate in the bacterial degradation of atropine.6-羟基环庚-1,4-二酮作为阿托品细菌降解过程中一种新型中间体的分离与鉴定。
Biochem J. 1993 Jul 1;293 ( Pt 1)(Pt 1):115-8. doi: 10.1042/bj2930115.
2
Tropine dehydrogenase: purification, some properties and an evaluation of its role in the bacterial metabolism of tropine.托品脱氢酶：纯化、某些特性及其在托品细菌代谢中作用的评估
Biochem J. 1995 Apr 15;307 ( Pt 2)(Pt 2):603-8. doi: 10.1042/bj3070603.
3
Atropine Metabolism by Pseudomonas sp. Strain AT3: Evidence for Nortropine as an Intermediate in Tropine Breakdown and Reactions Leading to Succinate.铜绿假单胞菌 AT3 株对阿托品的代谢：颠茄碱作为托品降解中间产物的证据及导致琥珀酸的反应。
Appl Environ Microbiol. 1996 Sep;62(9):3245-50. doi: 10.1128/aem.62.9.3245-3250.1996.
4
Enzymology of oxidation of tropic acid to phenylacetic acid in metabolism of atropine by Pseudomonas sp. strain AT3.铜绿假单胞菌AT3菌株在阿托品代谢过程中将托品酸氧化为苯乙酸的酶学研究。
J Bacteriol. 1997 Feb;179(4):1044-50. doi: 10.1128/jb.179.4.1044-1050.1997.
5
The metabolism of atropine in man.阿托品在人体内的代谢。
J Pharm Pharmacol. 1986 Oct;38(10):781-4. doi: 10.1111/j.2042-7158.1986.tb04494.x.
6
Determination of nitrogen-15 isotope fractionation in tropine: evaluation of extraction protocols for isotope ratio measurement by isotope ratio mass spectrometry.测定托品中的氮-15 同位素分馏：通过同位素比质谱法评估同位素比测量的提取方案。
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Tropinone reductases, enzymes at the branch point of tropane alkaloid metabolism.托品酮还原酶，托烷生物碱代谢分支点处的酶。
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8
[Determination of atropine, tropine and tropic acid in decomposed atropine solutions].[分解阿托品溶液中阿托品、托品碱和托品酸的测定]
Cesk Farm. 1962 May 5;11:199-205.
9
Atropine: a sensitive gas chromatography-mass spectrometry assay and prepharmacokinetic studies.阿托品：一种灵敏的气相色谱-质谱分析法及药代动力学前期研究
Agents Actions. 1981 Nov;11(5):520-31. doi: 10.1007/BF02004716.
10
Immunolocalisation of two tropinone reductases in potato (Solanum tuberosum L.) root, stolon, and tuber sprouts.两种托品酮还原酶在马铃薯（Solanum tuberosum L.）根、匍匐茎和块茎芽中的免疫定位
Planta. 2006 Dec;225(1):127-37. doi: 10.1007/s00425-006-0335-8. Epub 2006 Jul 15.

引用本文的文献

1
Atropine Metabolism by Pseudomonas sp. Strain AT3: Evidence for Nortropine as an Intermediate in Tropine Breakdown and Reactions Leading to Succinate.铜绿假单胞菌 AT3 株对阿托品的代谢：颠茄碱作为托品降解中间产物的证据及导致琥珀酸的反应。
Appl Environ Microbiol. 1996 Sep;62(9):3245-50. doi: 10.1128/aem.62.9.3245-3250.1996.
2
Proton-nuclear magnetic resonance analyses of the substrate specificity of a beta-ketolase from Pseudomonas putida, acetopyruvate hydrolase.恶臭假单胞菌β-酮醇酶（乙酰丙酮酸水解酶）底物特异性的质子核磁共振分析。
J Bacteriol. 1999 Aug;181(16):5051-9. doi: 10.1128/JB.181.16.5051-5059.1999.
3
Enzymology of oxidation of tropic acid to phenylacetic acid in metabolism of atropine by Pseudomonas sp. strain AT3.铜绿假单胞菌AT3菌株在阿托品代谢过程中将托品酸氧化为苯乙酸的酶学研究。
J Bacteriol. 1997 Feb;179(4):1044-50. doi: 10.1128/jb.179.4.1044-1050.1997.
4
Degradation of cocaine by a mixed culture of Pseudomonas fluorescens MBER and Comamonas acidovorans MBLF.荧光假单胞菌MBER和嗜酸丛毛单胞菌MBLF混合培养物对可卡因的降解作用。
Appl Environ Microbiol. 1996 Jan;62(1):94-9. doi: 10.1128/aem.62.1.94-99.1996.
5
Tropine dehydrogenase: purification, some properties and an evaluation of its role in the bacterial metabolism of tropine.托品脱氢酶：纯化、某些特性及其在托品细菌代谢中作用的评估
Biochem J. 1995 Apr 15;307 ( Pt 2)(Pt 2):603-8. doi: 10.1042/bj3070603.

本文引用的文献

1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
[On the degradation of atropine by Corynebacterium belladonnae. II].[关于颠茄棒状杆菌对阿托品的降解作用。II]
Hoppe Seylers Z Physiol Chem. 1961 Oct 25;326:9-12. doi: 10.1515/bchm2.1961.326.1.9.
3
[On the decomposition of atropine by Corynebacterium belladonnae].[关于颠茄棒状杆菌对阿托品的分解作用]
Hoppe Seylers Z Physiol Chem. 1959;317:238-42. doi: 10.1515/bchm2.1959.317.1.238.
4
[Bacteria decomposing tropane alkaloids].[分解托烷生物碱的细菌]
Acta Microbiol Pol (1952). 1960;9:173-9.
5
The breakdown of tropic acid in Pseudomonas putida strain L. I. Utilization of various substrates; the conversion of tropic acid into phenylacetic acid.恶臭假单胞菌L.I.菌株中托品酸的分解代谢。各种底物的利用；托品酸向苯乙酸的转化。
Biochim Biophys Acta. 1971 Feb 23;230(2):204-11. doi: 10.1016/0304-4165(71)90205-4.
6
The isolation and properties of Pseudomonas strains growing on atropine and producing atropinesterase.在阿托品上生长并产生阿托品酯酶的假单胞菌菌株的分离及特性
Proc K Ned Akad Wet C. 1971;74(2):132-47.

6-羟基环庚-1,4-二酮作为阿托品细菌降解过程中一种新型中间体的分离与鉴定。

The isolation and identification of 6-hydroxycyclohepta-1,4-dione as a novel intermediate in the bacterial degradation of atropine.

作者信息

Bartholomew B A, Smith M J, Long M T, Darcy P J, Trudgill P W, Hopper D J

机构信息

Department of Biochemistry, University of Wales, Aberystwyth, Dyfed, U.K.

出版信息

Biochem J. 1993 Jul 1;293 ( Pt 1)(Pt 1):115-8. doi: 10.1042/bj2930115.

DOI:10.1042/bj2930115
PMID:8328951
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1134327/
Abstract

Growth of Pseudomonas AT3 on the alkaloid atropine as its sole source of carbon and nitrogen is nitrogen-limited and proceeds by degradation of the tropic acid part of the molecule, with the metabolism of the tropine being limited to the point of release of its nitrogen. A nitrogen-free compound accumulated in the growth medium and was isolated and identified as 6-hydroxycyclohepta-1,4-dione. This novel compound is proposed as an intermediate in tropine metabolism. It served as a growth substrate for the organism and was also the substrate for an NAD(+)-linked dehydrogenase present in cell extracts. The enzyme was induced during the tropine phase of diauxic growth on atropine or during growth on tropine alone.

摘要

铜绿假单胞菌AT3在以生物碱阿托品作为唯一碳源和氮源的条件下生长时，受氮限制，其生长过程是通过降解分子中的托品酸部分进行的，而托品碱的代谢仅限于其氮释放的阶段。一种无氮化合物在生长培养基中积累，经分离和鉴定为6-羟基环庚-1,4-二酮。这种新化合物被认为是托品碱代谢的中间产物。它可作为该生物体的生长底物，也是细胞提取物中一种与NAD(+)相关的脱氢酶的底物。该酶在阿托品双相生长的托品碱阶段或仅在托品碱上生长时被诱导产生。