Ottolenghi A, Weatherly N F, Kocan A A, Larsh J E
Infect Immun. 1977 Jan;15(1):13-8. doi: 10.1128/iai.15.1.13-18.1977.
Rats given an initial infection with Angiostrongylus cantonensis had moderately elevated phospholipase B activity in the lungs at 8 and 15 days after challenge, and greatly elevated levels were evident at 35, 43, and 49 days. In the brain, the values were elevated at 15 through 35 days. These periods of increased activity in the lungs and brain coincided with the migration patterns of the third stage larvae and the adult worms in this host. The elevated enzyme levels also were were correlated with increased numbers of eosinophils in the bone marrow at 8 and 15 days and again at 36, 43, and 49 days after infection. Similarly infected rats exhibited leukocytosis at 1 through 10 weeks of observation after challenge, and striking eosinophilia at 1, 7, 8, and 9 weeks. Rats reinfected after removal of the worms of the initial infection by thiabendazole treatment showed an anamnestic response characterized by (i) elevated enzyme values in both the lungs and brain at 1 day after reinfection and (ii) eosinophilia in the bone marrow by day 4. These accelerated responses were accompanied by a significant reduction in the worm burden of the rats. The results, which support our hypothesis that inflammation, elevated phospholipase B activity, and reduction in worm burden are causally related, are discussed in light of similar findings reported earlier from our studies with Trichinella spiralis and Nippostrongylus brasiliensis.
初次感染广州管圆线虫的大鼠，在攻击后8天和15天时肺中的磷脂酶B活性中度升高，在35天、43天和49天时明显大幅升高。在脑中，酶活性值在15天至35天期间升高。肺和脑中这些活性增加的时期与该宿主中第三期幼虫和成虫的迁移模式相吻合。酶水平升高还与感染后8天和15天以及36天、43天和49天时骨髓中嗜酸性粒细胞数量增加相关。同样感染的大鼠在攻击后1至10周的观察期内出现白细胞增多，在第1、7、8和9周时出现明显的嗜酸性粒细胞增多。经噻苯达唑治疗清除初次感染的蠕虫后再次感染的大鼠表现出回忆反应，其特征为：（i）再次感染后1天肺和脑中的酶值升高；（ii）到第4天时骨髓中出现嗜酸性粒细胞增多。这些加速反应伴随着大鼠体内蠕虫负荷的显著降低。结合我们早期对旋毛虫和巴西日圆线虫研究报告的类似发现，对支持我们的炎症、磷脂酶B活性升高和蠕虫负荷降低存在因果关系这一假设的结果进行了讨论。
