Veselá J, Rehák P, Baran V, Koppel J
Institute of Animal Physiology, Slovak Academy of Sciences, Koŝice.
Physiol Res. 1993;42(1):23-7.
To estimate the significance of insulin in the regulation of preimplantation embryo growth, female mice received a single subdiabetogenic dose of streptozocin (65 mg/kg intraperitoneally) 8-11 days or 14-17 days before fertilization. Mean glycaemia levels and the number of embryos per mouse did not differ significantly between the streptozocin-treated and control groups. Morphological analysis of preimplantation embryos collected on day 3 of pregnancy revealed significant changes in the distribution pattern of preimplantation embryo stages recovered from streptozocin-treated females. Continuous insulin treatment of streptozocin-treated mice improved the impaired development of preimplantation embryos only in short-lasting experiments. After a long subdiabetic period (14-17 days) the incidence of degenerated embryos was increased in both streptozocin-treated groups. It can be concluded that the subdiabetic state in female mice impairs preimplantation embryo development which could partly be prevented by insulin treatment.
为评估胰岛素在着床前胚胎生长调节中的重要性，在受精前8 - 11天或14 - 17天，给雌性小鼠腹腔注射单次低于致糖尿病剂量的链脲佐菌素（65 mg/kg）。链脲佐菌素处理组和对照组之间，平均血糖水平和每只小鼠的胚胎数量无显著差异。对妊娠第3天收集的着床前胚胎进行形态学分析，结果显示，从链脲佐菌素处理的雌性小鼠回收的着床前胚胎阶段的分布模式有显著变化。仅在短期实验中，对链脲佐菌素处理的小鼠进行持续胰岛素治疗可改善着床前胚胎的发育受损情况。在长期处于低于糖尿病状态（14 - 17天）后，两个链脲佐菌素处理组中退化胚胎的发生率均增加。可以得出结论，雌性小鼠的低于糖尿病状态会损害着床前胚胎发育，胰岛素治疗可部分预防这种损害。
