Benghuzzi H A, Bajpai P K, England B G
Department of Pathology, University of Michigan, Ann Arbor 48109.
Biomed Sci Instrum. 1993;29:51-8.
The present study was undertaken with the purpose of investigating the effectiveness of CDS implants to deliver progesterone (P) and estradiol (E) in a sustained levels for long duration using adult female rats as a model. A total of 128 CDS implants, each having a final fired density of 1.88 +/- S.D. 0.17 g/cm3, were fabricated by using standard laboratory procedure. A total of 80 CD-female rats were randomly divided into five equal groups. Rats in groups II, III and IV and V were implanted with capsules containing 100 mg P, 5 mg E, 100 mg P plus 5 mg E, and 100 mg P plus 10 mg E. Group I animals were left without implants and served as controls. At the end of 2,4, 6 and 10 weeks postimplantation four rats from each group were euthanized and the reproductive organs were collected and the CDS devices were retrieved. The total amount of P and E left within the devices was determined spectrophotometrically. Data obtained indicates that a sustained rather constant release of P and E during the entire investigation. Steroid delivery rates from the CDS implants in groups II and III and IV differed significantly (P,0.05) for the 10 week period of the experiment. The results show that the rate of E released from the CDS implants was directly proportional to the amount of steroid placed initially in the reservoir.(ABSTRACT TRUNCATED AT 250 WORDS)
本研究旨在以成年雌性大鼠为模型，研究控释系统（CDS）植入物长期持续释放孕酮（P）和雌二醇（E）的有效性。采用标准实验室程序制作了总共128个CDS植入物，每个植入物的最终烧结密度为1.88±标准差0.17 g/cm³。总共80只成年雌性大鼠被随机分为五组。第二、三、四和五组的大鼠分别植入含有100 mg P、5 mg E、100 mg P加5 mg E和100 mg P加10 mg E的胶囊。第一组动物不植入任何东西，作为对照。在植入后2、4、6和10周结束时，每组处死4只大鼠，收集生殖器官并取出CDS装置。用分光光度法测定装置内剩余的P和E的总量。获得的数据表明，在整个研究过程中，P和E持续且相对稳定地释放。在实验的10周期间，第二、三、四组CDS植入物的类固醇释放率有显著差异（P<0.05）。结果表明，CDS植入物释放E的速率与最初置于储库中的类固醇量成正比。（摘要截断于250字）
