去势和睾酮替代对大鼠下丘脑酪氨酸羟化酶活性的影响。
Influence of castration and testosterone replacement on hypothalamic tyrosine hydroxylase activity in the rat.
作者信息
Beattie C W, Martin M M
出版信息
Experientia. 1976 Aug 15;32(8):1076-7. doi: 10.1007/BF01933981.
PMID:8330
Abstract
Hypothalamic tyrosine hydroxylase (TH) activity of castrate rats is modulated by testosterone propionate (TP) in vivo. Kinetic studies revealed that both Vmax and Km were virtually unaltered for substrate tyrosine in the presence of an excess of DMPH4 cofactor. TP replacement to castrate rats increased the Km for added DMPH4 cofactor, while Vmax decreased. These results suggest that TP decreases TH activity of castrate rats by inhibiting the enzyme-reduced pteridine cofactor complex.
摘要
去势大鼠下丘脑酪氨酸羟化酶（TH）的活性在体内受到丙酸睾酮（TP）的调节。动力学研究表明，在过量的DMPH4辅因子存在下，底物酪氨酸的Vmax和Km实际上没有改变。给去势大鼠补充TP会增加添加的DMPH4辅因子的Km，而Vmax降低。这些结果表明，TP通过抑制酶-还原型蝶啶辅因子复合物来降低去势大鼠的TH活性。
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