Ohyumi M, Takano S
Histochemistry. 1977 Jan 24;50(3):239-50. doi: 10.1007/BF00491071.
Ultrastructural and histochemical studies on human gastric cancer cells disclosed the presence of native and synthesized glycogen particles. The glycogen particles were investigated in the histochemical synthesis of glycogen particles from glucose 1-phosphate by the phosphorylase-branching glycosyltransferase system and non-incubated native glycogen in human gastric adenocarcinoma tubulare. It was observed that focal synthesis localized inthe intracytoplasmic matrix and intranucleus. Intranuclear synthesized glycogen appeared as a rosette form ranging from 1100 to 1300 A in diameter and free particles ranging from 325 to 900 A in diagmeter. The synthesis of glycogen appeared in the nucleus as well as in the cytoplasm of the human gastric cancer cells, and the synthesized glycogen was observed as a group of particles. Newly formed glycogen particles appeared occasionally in the interchromatin area as a large macromolecular structure of rosette form. Native glycogen appeared as a free-particle (250-333 A, medium =300 A) and aggregated rosette from (694-1050 A, medium=917 A) in the autophagosome of gastric cancer cells. There was not, however, a native glycogen particle in the nuclei of gastric cancer cells. Under certain conditons the nuclei of gastric cancer cells can acquire the capacity to synthesize glycogen.
对人类胃癌细胞的超微结构和组织化学研究揭示了天然和合成糖原颗粒的存在。在人胃腺癌管状组织中，通过磷酸化酶-分支糖基转移酶系统从1-磷酸葡萄糖进行糖原颗粒的组织化学合成以及未孵育的天然糖原中对糖原颗粒进行了研究。观察到局灶性合成定位于胞质基质和细胞核内。核内合成的糖原呈直径为1100至1300埃的玫瑰花结形式以及直径为325至900埃的游离颗粒。糖原的合成出现在人胃癌细胞的细胞核以及细胞质中，并且合成的糖原被观察为一组颗粒。新形成的糖原颗粒偶尔出现在染色质间区域，呈玫瑰花结形式的大分子结构。天然糖原在胃癌细胞的自噬体中呈游离颗粒（250 - 333埃，平均 = 300埃）和聚集的玫瑰花结形式（694 - 1050埃，平均 = 917埃）。然而，胃癌细胞的细胞核中不存在天然糖原颗粒。在某些条件下，胃癌细胞的细胞核能够获得合成糖原的能力。
