Troyer H, Fisher O T, Rosenquist T H
Histochemistry. 1977 Jan 24;50(3):251-9. doi: 10.1007/BF00491072.
Studies of enzyme kinetics by histochemical methods has been seriously impaired by available methods. This study combines two newer methods, i.e., sectioning undecalcified bone from glycol methacrylate and the use of atomic absorpton spectrophotometry for photographic densitometry. The results indicate that the substrate concentration for zero order kinetics of the alkaline phosphatase reaction in bone is 0.5 mg/ml of substrate; and the Km of the same reaction is estimated to be 0.25 mg/ml or 0.56 mM of substrate.
利用组织化学方法对酶动力学进行的研究一直受到现有方法的严重影响。本研究结合了两种更新的方法，即从甲基丙烯酸乙二醇酯中切割未脱钙的骨切片以及使用原子吸收分光光度法进行图像密度测定。结果表明，骨中碱性磷酸酶反应零级动力学的底物浓度为0.5mg/ml底物；相同反应的米氏常数估计为0.25mg/ml或0.56mM底物。
