Benkö Z, Sipiczki M
Department of Genetics, University of Debrecen, Hungary.
Can J Microbiol. 1993 May;39(5):551-4. doi: 10.1139/m93-079.
Caffeine at concentrations of 8 mM or higher inhibited cell propagation and killed a fraction of the population. Cell inactivation increased incrementally with increasing concentrations. The survivors developed tolerance by physiological adaptation that enabled them to propagate in the presence of the drug, but the tolerance could easily be lost if the cells grew in the absence of caffeine for a few generations. Theophylline was found to diminish the toxic effect of caffeine. Possible mechanisms for the observed cellular response and its implications for studies of the effects of these drugs in eukaryotes are discussed.
浓度为8 mM或更高的咖啡因会抑制细胞增殖，并杀死一部分细胞群体。细胞失活随着浓度的增加而逐渐增加。存活下来的细胞通过生理适应产生了耐受性，使它们能够在药物存在的情况下增殖，但如果细胞在没有咖啡因的情况下生长几代，这种耐受性很容易丧失。研究发现茶碱可以减轻咖啡因的毒性作用。本文讨论了观察到的细胞反应的可能机制及其对这些药物在真核生物中作用研究的意义。
