Renshaw H W, Vaughn H W, Magonigle R A, Davis W C, Stauber E H, Frank F W
J Am Vet Med Assoc. 1977 Feb 1;170(3):334-9.
Samples of blood from 87 Rocky Mountain mule deer (Odocoileus hemionus hemionus) were inoculated into 3 susceptible splenectomized calves to determine the anaplasmosis carrier status of the deer. The deer were trapped along the Idaho-Utah state boundary, near Stone, Id. Acute anaplasmosis was induced in 2 of the 3 inoculated calves, and blood from the 3 calves caused anaplasmosis when inoculated into adult non-splenectomized cattle. Serum rapid card agglutination testing revealed 13 (14.9%) positive reactions among the 87 mule deer. When these seropositive samples were evaluated with the complement-fixation test, positive reactions were not found and only 3 (3.5%) serums gave suspect reactions. A serologic survey for anaplasmosis in 1,852 cattle that cohabit spring and summer rangeland areas with mule deer revealed 14.5% reactors by the serum rapid card agglutination test. The study area consisted of an ecosystem that supports cattle, free-roaming mule deer, and Dermacentor andersoni, and thus contained the elements necessary to effect interspecies transmission of Anaplasma marginale. The significance of wildlife reservoirs and their bearing on anaplasmosis control and eradication programs needs further investigation.
采集了87只落基山骡鹿（Odocoileus hemionus hemionus）的血样，接种到3头易感的脾切除小牛体内，以确定这些鹿的无形体病携带状态。这些鹿是在爱达荷州与犹他州州界附近、爱达荷州斯通附近捕获的。3头接种的小牛中有2头发病，出现急性无形体病，这3头小牛的血液接种到成年非脾切除牛体内后也引发了无形体病。血清快速卡片凝集试验显示，87只骡鹿中有13只（14.9%）呈阳性反应。对这些血清学阳性样本进行补体结合试验评估时，未发现阳性反应，只有3份（3.5%）血清出现可疑反应。对与骡鹿在春夏牧场共同生活的1852头牛进行无形体病血清学调查，通过血清快速卡片凝集试验发现14.5%的牛呈阳性反应。研究区域是一个支持牛、自由放养的骡鹿和安氏革蜱生存的生态系统，因此具备实现边缘无形体种间传播所需的要素。野生动物宿主的重要性及其对无形体病防控和根除计划的影响需要进一步研究。
