Samaan G J, Porquet D, Demelier J F, Biou D
Service de Biochimie-Hormonologie, Hôpital R. Debre, Paris, France.
Clin Biochem. 1993 Jun;26(3):153-8. doi: 10.1016/0009-9120(93)90019-3.
We describe a method for the determination of urinary free cortisol and glucocorticoids in plasma, used in the diagnosis of adrenal disorders, based on automated reverse-phase high-performance liquid chromatography (HPLC). The within-day and day-to-day CVs were less than 5.5 and 8.0%, respectively. The calibration curves for cortisol and 11-deoxycortisol were linear up to 2000 nmol/L. Cortisol concentrations as low as 3.5 nmol/L in 1 mL of plasma or urine can be measured. Correlation of HPLC results for 40 plasma specimens with those by radioimmunoassay showed r = 0.965. This method is sensitive and free from the interference habitually encountered in immunoassays, and can thus be proposed for research and as a potential reference method.
我们描述了一种基于自动反相高效液相色谱法（HPLC）测定尿液游离皮质醇和血浆糖皮质激素的方法，用于肾上腺疾病的诊断。日内和日间变异系数分别小于5.5%和8.0%。皮质醇和11-脱氧皮质醇的校准曲线在高达2000 nmol/L时呈线性。在1 mL血浆或尿液中低至3.5 nmol/L的皮质醇浓度都可以测量。40份血浆样本的HPLC结果与放射免疫分析结果的相关性显示r = 0.965。该方法灵敏，且不存在免疫分析中常见的干扰问题，因此可用于研究并作为一种潜在的参考方法。
