Webb L E, Pavlina T M, Hjelle J T
Baxter Healthcare Corporation, Round Lake, IL.
Clin Biochem. 1993 Jun;26(3):173-7. doi: 10.1016/0009-9120(93)90022-x.
Mesothelial cells lining the peritoneal cavity utilize choline in the synthesis of a phosphatidylcholine-rich material thought to play a role in peritoneal homeostasis. This function is particularly important for patients undergoing continuous ambulatory peritoneal dialysis (CAPD). To assess choline loss in these patients, we measured choline in plasma and peritoneal dialysis effluent (PDE) by a rapid high performance liquid chromatography (HPLC) procedure that combined electrochemical detection with an immobilized enzyme reactor. Chromatography was performed directly on plasma and PDE ultrafiltrates. In 30 patients, the amount of choline lost to the dialysate was 129 +/- 49 mumol per day and 32 +/- 8 mumol per dwell (mean +/- SD). The average plasma choline concentration was 22.5 mumol/L, a value somewhat higher than the mean value reported for normal adults (9 mumol/L). The average PDE choline concentration was 14 mumol/L. There was a positive correlation between daily choline loss of dialysate and plasma choline concentrations (r = 0.826).
衬于腹膜腔的间皮细胞在合成一种富含磷脂酰胆碱的物质时会利用胆碱，该物质被认为在腹膜内环境稳定中发挥作用。这一功能对于接受持续性非卧床腹膜透析（CAPD）的患者尤为重要。为评估这些患者的胆碱损失情况，我们采用一种快速高效液相色谱（HPLC）方法测定血浆和腹膜透析流出液（PDE）中的胆碱，该方法将电化学检测与固定化酶反应器相结合。直接对血浆和PDE超滤物进行色谱分析。在30例患者中，每日进入透析液的胆碱损失量为129±49μmol，每次驻留损失量为32±8μmol（均值±标准差）。血浆胆碱平均浓度为22.5μmol/L，该值略高于正常成年人报告的均值（9μmol/L）。PDE胆碱平均浓度为14μmol/L。透析液每日胆碱损失量与血浆胆碱浓度之间存在正相关（r = 0.826）。
