Breborowicz A, Patrikarea A, Martis L, Oreopoulos D G
Department of Pathophysiology, Medical School of Poznan, Poland.
Adv Perit Dial. 1994;10:230-4.
The authors studied the in vitro effect of overnight "dwelled" dialysate obtained from 5 continuous ambulatory peritoneal dialysis (CAPD) patients on the growth of, and protein synthesis by, peritoneal mesothelial cells and fibroblasts. In each patient, an overnight exchange was performed with Dianeal 2.5% on day 1 and with a solution containing amino acids 1.1% on day 2. Both types of dialysate supported the proliferation of fibroblasts and mesothelial cells, but the amino acid dialysate had a stronger effect on the growth of mesothelial cells than the Dianeal dialysate did. In the presence of either dialysate, synthesis of noncollagen proteins by mesothelial cells and fibroblasts was low. By contrast, the synthesis of collagen was enhanced, and the Dianeal dialysate had a much stronger effect than the amino acid dialysate did. Present results show that, during its intraperitoneal dwell, dialysis fluid within the peritoneal cavity stimulates the generation of bioactive substances which affect the function of peritoneal cells. This reaction is influenced by the composition of the dialysis solution.
作者研究了从5名持续性非卧床腹膜透析（CAPD）患者获取的过夜“留存”透析液对腹膜间皮细胞和成纤维细胞生长及蛋白质合成的体外作用。在每位患者中，第1天用2.5%的 Dianeal 进行过夜换液，第2天用含1.1%氨基酸的溶液进行换液。两种类型的透析液均支持成纤维细胞和间皮细胞的增殖，但氨基酸透析液对间皮细胞生长的作用比 Dianeal 透析液更强。在任何一种透析液存在的情况下，间皮细胞和成纤维细胞的非胶原蛋白合成均较低。相比之下，胶原蛋白的合成增强，且 Dianeal 透析液的作用比氨基酸透析液强得多。目前的结果表明，腹膜腔内的透析液在其腹腔内留存期间会刺激生物活性物质的产生，这些物质会影响腹膜细胞的功能。这种反应受透析液成分的影响。
