Coexistence of tubulin, vimentin and F-actin in Leydig cells in vitro detected by double immunofluorescence studies.

Double indirect immunofluorescence using antibodies against tubulin, vimentin and/or F-actin, have shown that microtubules (MT), vimentin intermediate filaments (IF) and microfilaments can be simultaneously labelled in cultured mouse Leydig cells. The results indicate that at the level of resolution of the fluorescence microscope there is an extensive but not complete superposition of the filamentous distributions of the labels for tubulin/F-actin and vimentin/F-actin during interphase, suggesting that extensive association of the two filaments exists in these cells. Visible juxtaposition is evident between MT and vimentin IF.