Pringle N P, Richardson W D
Department of Biology, University College London, UK.
Development. 1993 Feb;117(2):525-33. doi: 10.1242/dev.117.2.525.
During rat embryogenesis, PDGF alpha receptor (PDGF-alpha R) mRNA is expressed in the ventral half of the spinal cord in two longitudinal columns, one each side of the central canal. Initially, these columns are only two cells wide but the cells subsequently appear to proliferate and disseminate throughout the spinal cord. Our previous studies of PDGF-alpha R expression in the developing CNS suggested that PDGF-alpha R may be a useful marker of the oligodendrocyte lineage in situ. The data presented here complement those studies and lead us to propose that the earliest oligodendrocyte precursors in the spinal cord originate in a very restricted region of the ventricular zone during a brief window of time around embryonic day 14 (E14). In the embryonic brain, migrating PDGF-alpha R+ cells appear to originate in a localized germinal zone in the ventral diencephalon (beneath the foramen of Monro). Our data demonstrate that gene expression and cell fate can be regulated with exquisite spatial resolution along the dorsoventral axis of the mammalian neural tube.
在大鼠胚胎发育过程中，血小板衍生生长因子α受体（PDGF-αR）mRNA在脊髓腹侧半部的两个纵向柱状结构中表达，中央管两侧各有一个。最初，这些柱状结构只有两个细胞宽，但随后细胞似乎增殖并扩散到整个脊髓。我们之前对发育中的中枢神经系统中PDGF-αR表达的研究表明，PDGF-αR可能是原位少突胶质细胞谱系的一个有用标记。此处呈现的数据补充了那些研究，并使我们提出，脊髓中最早的少突胶质细胞前体在胚胎第14天（E14）左右的短暂时间窗口内起源于室带的一个非常有限的区域。在胚胎大脑中，迁移的PDGF-αR+细胞似乎起源于腹侧间脑（在孟氏孔下方）的一个局部生发区。我们的数据表明，基因表达和细胞命运可以沿着哺乳动物神经管的背腹轴以精确的空间分辨率进行调控。
