胎儿三毛滴虫肋的分离及生化特性研究
Isolation and biochemical characterization of the costa of Tritrichomonas foetus.
作者信息
Monteiro-Leal L H, da Cunha-e-Silva N L, Benchimol M, de Souza W
机构信息
Laboratorio de Ultraestrutura Celular Hertha Meyer, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Brasil.
出版信息
Eur J Cell Biol. 1993 Apr;60(2):235-42.
The costae are cytoskeletal structures found in Trichomonadidae. Both the structural organization and composition of this organelle are still unknown. In the present work we have introduced a new methodology for the costa isolation. Using sucrose density-gradient centrifugation an enriched costa fraction was obtained. Analyses by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed that the costa contains several proteins, with major bands corresponding to apparent molecular masses of 122, 115, 112, 93, 87, 82, 59, 52, 44, 41, 32, and 26 kDa. No significant amount of carbohydrates was detected in the costa fraction. The fractionation methodology described here has the advantage of using normal centrifugation methods and is being applied to trichomonas in the size range of Tritrichomonas foetus and Trichomonas vaginalis.
肋是在毛滴虫科中发现的细胞骨架结构。该细胞器的结构组织和组成仍然未知。在本研究中，我们引入了一种新的肋分离方法。通过蔗糖密度梯度离心获得了富含肋的组分。十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）分析表明，肋含有几种蛋白质，主要条带对应的表观分子量为122、115、112、93、87、82、59、52、44、41、32和26 kDa。在肋组分中未检测到大量碳水化合物。这里描述的分级分离方法具有使用常规离心方法的优点，并且正在应用于胎儿三毛滴虫和阴道毛滴虫大小范围内的滴虫。
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