Clementi E, Raichman M, Meldolesi J
Chair of Pharmacology, Faculty of Pharmacy, University of Reggio di Calabria, Catanzaro, Italy.
Funct Neurol. 1993 Mar-Apr;8(2):109-13.
The effect of NGF on a set of 17 PC12 cell clones was investigated in order to clarify some aspects concerning the changes in cell morphology and Ca2+ homeostasis that occur in the PC12 cell line after NGF-induced differentiation. The morphological parameters analyzed showed a high degree of heterogeneity among clones, consisting with a different expression of the high-affinity NGF receptor. The NGF effect on Ca2+ influx was investigated in a clone highly sensitive to the cholinergic agonist carbachol. Results showed an increase in Ca2+ influx through one of the carbachol-activated Ca2+ channels.
为了阐明有关神经生长因子（NGF）诱导分化后PC12细胞系中细胞形态和Ca2+稳态变化的某些方面，研究了NGF对一组17个PC12细胞克隆的影响。分析的形态学参数显示克隆间存在高度异质性，这与高亲和力NGF受体的不同表达一致。在对胆碱能激动剂卡巴胆碱高度敏感的一个克隆中研究了NGF对Ca2+内流的影响。结果显示通过其中一个卡巴胆碱激活的Ca2+通道的Ca2+内流增加。
