Hasegawa T, Tsuji M, Nakayama S, Oguchi K
Department of Pharmacology, School of Medicine, Showa University, Tokyo, Japan.
Nihon Yakurigaku Zasshi. 1993 May;101(5):337-47. doi: 10.1254/fpj.101.5_337.
The effects of formaldehyde (F), m-cresol (C), guaiacol (G), ethanol (E) and their mixture (FC, FCE, FG, FGE) on erythrocytes and isolated hepatocytes from rats and surface tension in water were examined. Hypotonic hemolysis of erythrocytes was inhibited by m-cresol, while guaiacol, formaldehyde and ethanol accelerated the hemolysis. Lower concentrations of the mixture inhibited hypotonic hemolysis, but higher concentrations accelerated hemolysis. Formaldehyde caused a decrease of transaminase (GOT, GPT) in the medium and hepatocytes. GOT and GPT in the medium were increased by m-cresol, but those in the hepatocyte were decreased by this agent. FC and FCE at 10 mM increased GOT in the medium, but FG and FGE decreased GOT. All mixtures decreased GOT and GPT in hepatocytes and GPT in the medium. All mixtures and formaldehyde inhibited GOT and GPT activity. Formaldehyde and m-cresol decreased hepatocyte viability. In the all mixtures-added hepatocytes, the viability was markedly lowered. Formaldehyde, m-cresol, guaiacol and ethanol caused a depression of surface tension, but the depressive effects of FG and FGE were weaker than that of guaiacol. These results suggest that the observed effects of the drug mixtures on erythrocytes and hepatocytes were the additive effects of the component drugs.
研究了甲醛（F）、间甲酚（C）、愈创木酚（G）、乙醇（E）及其混合物（FC、FCE、FG、FGE）对大鼠红细胞、离体肝细胞以及水表面张力的影响。间甲酚可抑制红细胞的低渗溶血，而愈创木酚、甲醛和乙醇则加速溶血。较低浓度的混合物可抑制低渗溶血，但较高浓度则加速溶血。甲醛可使培养基和肝细胞中的转氨酶（谷草转氨酶、谷丙转氨酶）降低。间甲酚可使培养基中的谷草转氨酶和谷丙转氨酶升高，但可使肝细胞中的这两种酶降低。10 mM的FC和FCE可使培养基中的谷草转氨酶升高，但FG和FGE则使其降低。所有混合物均可使肝细胞中的谷草转氨酶和谷丙转氨酶以及培养基中的谷丙转氨酶降低。所有混合物和甲醛均可抑制谷草转氨酶和谷丙转氨酶的活性。甲醛和间甲酚可降低肝细胞活力。在添加所有混合物的肝细胞中，活力明显降低。甲醛、间甲酚、愈创木酚和乙醇均可降低表面张力，但FG和FGE的降低作用弱于愈创木酚。这些结果表明，所观察到的药物混合物对红细胞和肝细胞的影响是各成分药物的相加作用。
