Fan G, Carbajo P, Rillema J A
Department of Physiology, Wayne State University School of Medicine, Detroit, Michigan.
Horm Metab Res. 1993 May;25(5):256-8. doi: 10.1055/s-2007-1002091.
Orthovanadate and genistein were employed to determine the possible role of tyrosine kinase in the prolactin stimulation of cell division in Nb2 node lymphoma cells. Orthovanadate (0.1-0.25 microM), an inhibitor of specific phosphatases, was found to stimulate cell division as well as potentiate the mitogenic effect of prolactin on Nb2 cells when prolactin was employed at a less than maximum stimulatory concentration. Genistein, an inhibitor of tyrosine kinase, was found to inhibit the prolactin stimulation of cell division. Since prolactin was shown in earlier studies to stimulate the phosphorylation of tyrosyl residues in specific Nb2 cell proteins, these results suggest that the signal transduction pathway for the prolactin stimulation of cell division in the Nb2 node lymphoma cells likely involves the participation of tyrosine kinase(s).
用原钒酸盐和染料木黄酮来确定酪氨酸激酶在Nb2结节淋巴瘤细胞中催乳素刺激细胞分裂过程中可能发挥的作用。原钒酸盐（0.1 - 0.25微摩尔）是一种特异性磷酸酶抑制剂，当以低于最大刺激浓度使用催乳素时，发现它能刺激细胞分裂，并增强催乳素对Nb2细胞的促有丝分裂作用。染料木黄酮是一种酪氨酸激酶抑制剂，发现它能抑制催乳素对细胞分裂的刺激作用。由于在早期研究中已表明催乳素能刺激Nb2细胞特定蛋白质中酪氨酸残基的磷酸化，这些结果表明，Nb2结节淋巴瘤细胞中催乳素刺激细胞分裂的信号转导途径可能涉及酪氨酸激酶的参与。
