Molès J P, Theillet C, Basset-Sèguin N, Guilhou J J
Laboratoire de Dermatologie Moléculaire, CNRS/CRBM, Montpellier, France.
J Invest Dermatol. 1993 Jul;101(1):100-2. doi: 10.1111/1523-1747.ep12360920.
We investigated the tumor-suppressor gene, TP53, in psoriatic skin at gene (GP53) and protein (p53) levels. No mutation was detected in the seven exons analyzed using a combination of polymerase chain reaction and single-strand conformation polymorphism techniques. In addition, by immunohistochemistry using three different anti-p53 antibodies, no positive staining, reported to be associated with mutations and/or abnormal expression of the TP53 gene, was observed in psoriatic skin biopsies, in contrast to recent reports. These results suggest that the proliferative status of psoriatic keratinocytes does not implicate the TP53 gene.
我们在基因（GP53）和蛋白（p53）水平上研究了银屑病皮肤中的肿瘤抑制基因TP53。使用聚合酶链反应和单链构象多态性技术相结合的方法，在分析的七个外显子中未检测到突变。此外，与最近的报道相反，通过使用三种不同的抗p53抗体进行免疫组织化学检测，在银屑病皮肤活检中未观察到与TP53基因突变和/或异常表达相关的阳性染色。这些结果表明，银屑病角质形成细胞的增殖状态与TP53基因无关。
